Transformation ofMucor mieheiresults in plasmid deletion and phenotypic instability
نویسندگان
چکیده
منابع مشابه
Transformation of Mucor miehei results in plasmid deletion and phenotypic instability.
Mucor miehei transformants resistant to geneticin have been obtained by treatment of protoplasts with different plasmids and by Agrobacterium-mediated DNA transfer. All transformants exhibited a very unstable phenotype whose maintenance required continuous selective pressure. Molecular analysis of transformants showed that the plasmid DNA was extensively modified and maintained in very low amou...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2003
ISSN: 0378-1097,1574-6968
DOI: 10.1016/s0378-1097(03)00421-x